Human ADAM15 Ectodomain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF935
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM15 Ectodomain
Leu18-Ser693
Accession # Q13444
Leu18-Ser693
Accession # Q13444
Specificity
Detects human ADAM15 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) ADAM15 is observed, and less than 1% cross-reactivity with rmADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM9, rhADAM10, rhADAM12, rhADAM17 (TACE), rhADAM19, rhADAM22, rhADAM23, rhADAM32, and rhADAM33 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ADAM15 Ectodomain Antibody
ADAM15 in Human Prostate.
ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ADAM15 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF935) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human ADAM15 Ectodomain Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 2 using AF935 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM15
Long Name
A Disintegrin and Metalloprotease-like Domain 15
Alternate Names
MDC15, Metargidin
Gene Symbol
ADAM15
UniProt
Additional ADAM15 Products
Product Documents for Human ADAM15 Ectodomain Antibody
Product Specific Notices for Human ADAM15 Ectodomain Antibody
For research use only
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