Human ADAM15 Ectodomain Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF935
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM15
Met1-Ser693
Accession # Q13444
Met1-Ser693
Accession # Q13444
Specificity
Detects human ADAM15 in Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant mouse (rm) ADAM15 is observed and less than 1% cross‑reactivity with rmADAM9 and recombinant human ADAM8 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ADAM15 Ectodomain Biotinylated Antibody
ADAM15 in Human Prostate.
ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human ADAM15 Ectodomain Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF935) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human ADAM15 Ectodomain Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue and normal human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue and normal human prostate
Western Blot
0.1 µg/mL
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM15
Long Name
A Disintegrin and Metalloprotease-like Domain 15
Alternate Names
MDC15, Metargidin
Gene Symbol
ADAM15
UniProt
Additional ADAM15 Products
Product Documents for Human ADAM15 Ectodomain Biotinylated Antibody
Product Specific Notices for Human ADAM15 Ectodomain Biotinylated Antibody
For research use only
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