Human ADAM19 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5050
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAM19
Arg26-Gln501 (Ser32 del) (Lys33Arg)
Accession # Q9H013
Arg26-Gln501 (Ser32 del) (Lys33Arg)
Accession # Q9H013
Specificity
Detects human ADAM19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse ADAM19 is observed, and less than 5% cross-reactivity with recombinant human (rh) ADAM8, rhADAM12, and rhADAM33 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ADAM19 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM19, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM19, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human ADAM19
Sample: Recombinant Human ADAM19
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM19
ADAM19 (a disintegrin and metalloprotease 19; also MADDAM and Meltrin-beta) is a 95‑100 kDa member of the M12B peptidase family of enzymes. It is expressed on multiple cell types including monocytes, fibroblasts, osteoblasts and dendritic cells. The human ADAM19 proenzyme is a 931 amino acid (aa) type I transmembrane protein. It contains a 178 aa cleavable proregion (aa 26‑203) and a 497 aa extracellular domain (ECD) (aa 204‑702). The ECD contains a metalloprotease domain (aa 211‑409), a disintegrin region (aa 417‑503) and a Cys-rich domain (aa 504‑650). The ECD circulates in plasma bound to 700 kDa alpha2-Macroglobulin. One splice variant shows an alternate start site at Met71, while two isoforms show variations after Lys902. Over aa 205‑702, human ADAM19 is 87% aa identical to mouse ADAM19.
Long Name
A Disintegrin and Metalloprotease-like Domain 19
Alternate Names
Meltrin beta
Gene Symbol
ADAM19
UniProt
Additional ADAM19 Products
Product Documents for Human ADAM19 Antibody
Product Specific Notices for Human ADAM19 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...