Human ADAM23 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4974
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAM23
Ser60-His585
Accession # O75077
Ser60-His585
Accession # O75077
Specificity
Detects human ADAM23 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) ADAM8, rhADAM9, and rhADAM22 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ADAM23 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM23, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM23, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human ADAM23
Sample: Recombinant Human ADAM23
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM23
ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100 kDa member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum and serves as a counter‑receptor for alphav beta3 integrin. The human ADAM23 proprecursor is a 773 amino acid (aa) type I transmembrane (TM) protein. It contains a 227 aa cleavable proregion (aa 60‑286) and a 506 aa extracellular domain (ECD) (aa 287‑792) that is part of a 70 kDa mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299‑494), an integrin‑binding disintegrin region (aa 511‑585) and a Cys‑rich domain (aa 589‑611). Two splice variants exist. One shows an in‑frame 46 aa substitution for aa 787‑832 that generates a soluble form, while a second shows an in‑frame 31 aa TM substitution for aa 787‑817. Over aa 1‑585, human ADAM23 is 92% aa identical to mouse ADAM23.
Long Name
A Disintegrin and Metalloproteinase Domain 23
Alternate Names
MDC-L, MDC3
Gene Symbol
ADAM23
UniProt
Additional ADAM23 Products
Product Documents for Human ADAM23 Antibody
Product Specific Notices for Human ADAM23 Antibody
For research use only
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