Human ADAM32 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5380

R&D Systems, part of Bio-Techne
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MAB5380
MAB5380-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 539220

View all ADAM32 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM32
Ser17-Thr476
Accession # Q8TC27

Specificity

Detects human ADAM32 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ADAM32 Antibody

ADAM32 in MCF-7 Human Cell Line.

ADAM32 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ADAM32 Monoclonal Antibody (Catalog # MAB5380) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human ADAM32 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM32

ADAM32 (a disintegrin and metalloprotease domain 32) is a 98 kDa member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771 amino acid (aa) type I transmembrane protein. It contains a 158 aa proregion (aa 17‑174) and a 508 aa extracellular domain (ECD) (aa 175‑682). The ECD contains a nonfunctional metalloprotease domain (aa 186‑383), an integrin‑binding disintegrin region (aa 391‑479), a Cys‑rich segment (aa 480‑502) and an EGF‑like domain (aa 622‑654). In the testis, mature ADAM32 is 98 kDa in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44 kDa product. There are two potential splice events that show a deletion of aa 306‑401 plus a 53 aa substitution for the N‑terminal 46 amino acids. Over aa 17‑476, human ADAM32 is 66% aa identical to mouse ADAM32.

Long Name

A Disintegrin and Metalloproteinase Domain 32

Alternate Names

a disintegrin and metalloprotease domain 32, a disintegrin and metalloproteinase domain 32, ADAM 32, ADAM metallopeptidase domain 32, disintegrin and metalloproteinase domain-containing protein 32, FLJ26299, FLJ29004, metalloproteinase 12-like protein

Entrez Gene IDs

203102 (Human); 353188 (Mouse); 361170 (Rat)

Gene Symbol

ADAM32

UniProt

Q8TC27

Additional ADAM32 Products

Product Documents for Human ADAM32 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human ADAM32 Antibody

For research use only

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