Human ADAMTS8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6614
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS8
Pro29-Arg691 (Gly35Arg, Gly431Ala, Val526Ala)
Accession # AAD48081
Pro29-Arg691 (Gly35Arg, Gly431Ala, Val526Ala)
Accession # AAD48081
Specificity
Detects human ADAMTS8 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ADAMTS8 Antibody
Detection of Human ADAMTS8 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line either transfected with human ADAMTS8, mock transfected (-), or transfected with an unrelated vector. PVDF Membrane was probed with 1 µg/mL of Human ADAMTS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6614) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS8 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
ADAMTS8 in Human Brain.
ADAMTS8 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human ADAMTS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6614) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human ADAMTS8 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS8, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS8, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line either transfected with human ADAMTS8, mock transfected (-), or transfected with an unrelated vector
Sample: 293T human embryonic kidney cell line either transfected with human ADAMTS8, mock transfected (-), or transfected with an unrelated vector
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAMTS8
ADAMTS8 (A disintegrin and metalloprotease with thrombospondin motifs 8; also METH-2) is a 95 kDa member of an ADAMTS subfamily of Zn metalloproteases that includes ADAMST-1, -4, -5 and -15. It is expressed by chrondrocytes, neurons, astrocytes and macrophages, and likely participates in proteoglycan (aggrecan) proteolysis. Human proADAMTS8 is a secreted, 863 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 27-213), a peptidase M12B domain (aa 219-429), a disintegrin region (aa 438-525), and two TSP type I sequences (aa 526-888) that are separated by an intervening spacer domain (aa 690-831). Cleavage of the proregion generates a mature 80 kDa molecule that may undergo additional processing to create a 65-67 kDa truncated form. There are two potential splice variants. One shows a 31 aa substitution for aa 411-889, while another shows an alternative start site at Met231. Over aa 29-691, human ADAMTS8 shares 79% aa identity with mouse ADAMTS8.
Long Name
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 8
Alternate Names
METH-2, METH-8
Gene Symbol
ADAMTS8
UniProt
Additional ADAMTS8 Products
Product Documents for Human ADAMTS8 Antibody
Product Specific Notices for Human ADAMTS8 Antibody
For research use only
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