Human Adenosine A2aR/A2bR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB94972
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 599743
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Adenosine A2aR
Met1-Ser412
Accession # P29274
Met1-Ser412
Accession # P29274
Specificity
Detects human Adenosine A2aR and human Adenosine A2bR in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Adenosine A2aR/A2bR Antibody
Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human Adenosene A2aR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Detection of Adenosine A2bR in HEK293 Human Cell Line Transfected with Human Adenosine A2bR and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human Adenosene A2bR and eGFP was stained with (A) Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.Applications for Human Adenosine A2aR/A2bR Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Adenosine A2aR or Adenosine A2bR and eGFP
Sample: HEK293 Human Cell Line Transfected with Human Adenosine A2aR or Adenosine A2bR and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adenosine A2aR/A2bR
Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A1, A2A, A2B, and A3 have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A2A and A2B (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.
Long Name
Adenosine A2A Receptor/Adenosine A2B Receptor
UniProt
Additional Adenosine A2aR/A2bR Products
Product Documents for Human Adenosine A2aR/A2bR Antibody
Product Specific Notices for Human Adenosine A2aR/A2bR Antibody
For research use only
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