Human Adenosine A2b R Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10444
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1022311
Product Specifications
Immunogen
Synthetic peptide containing human Adenosine A2b R
Specificity
Detects human Adenosine A2b R in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Adenosine A2b R Antibody
Detection of Human Adenosine A2b R by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and human cerebeullum tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Adenosine A2b R Monoclonal Antibody (Catalog # MAB10444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adenosine A2b R at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Adenosine A2b R Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: A431 human epithelial carcinoma cell line and Human cerebeullum tissue
Sample: A431 human epithelial carcinoma cell line and Human cerebeullum tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adenosine A2b R
ADORA2B (Adenosine B2a Receptor, Adora-B2) is a widely expressed seven transmembrane G protein-coupled receptor (GPCR) activated by adenosine. Studies show that adenosine is crucial for ischemic preconditioning (IP) via its receptors ADORA1, ADORA2a and ADORA2b. All of these receptors are expressed in the myocardium and have been implicated in the role of cardioprotection during ischemia. Human and mouse Adora2B sequences are 88% identical over the full protein, but only 77% identical on the extra-cellular domains, including N-term tail and three loops.
Long Name
Adenosine A2b Receptor
Alternate Names
Adenosine A2bR, ADORA2B
Gene Symbol
ADORA2B
Additional Adenosine A2b R Products
Product Documents for Human Adenosine A2b R Antibody
Product Specific Notices for Human Adenosine A2b R Antibody
For research use only
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