Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6414

R&D Systems, part of Bio-Techne
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AF6414
AF6414-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all ADP-Sugar Pyrophosphatase/NUDT5 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ADP‑Sugar Pyrophosphatase/NUDT5
Glu2-Phe219
Accession # Q9UKK9

Specificity

Detects human ADP‑Sugar Pyrophosphatase/NUDT5 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody

Detection of Human ADP-Sugar Pyrophosphatase/ NUDT5 antibody by Western Blot.

Detection of Human ADP‑Sugar Pyrophosphatase/ NUDT5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ADP-Sugar Pyrophosphatase/NUDT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6414) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADP-Sugar Pyrophosphatase/NUDT5 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADP-Sugar Pyrophosphatase/NUDT5

ADP‑Sugar Pyrophosphatase (NUDT-5, Nucleoside diphosphate-linked moiety X motif 5; also YSA1H and 8-oxo-dGDP phosphatase) is a member of the Nudix hydrolase family of proteins. Although the predicted MW of NUDT-5 is 24 kDa, it runs anomalously at 32-36 kDa in SDS-Page. NUDT-5 is a widely expressed intracellular homodimer that has at least two functions. First, it possesses pyrophosphatase activity that prevents nonenzymatic ribosylation of proteins, and second, it hydrolyzes 8-oxoGDP (plus 8-OH-dADP), thus removing an oxidized nucleoside from the nucleotide pool and its potential for incorporation into either DNA or RNA. Such incorporation likely results in transcriptional or translational errors. Human NUDT-5 is 219 amino acids (aa) in length (SwissProt #:Q9UKK9). It is contains an N-terminal substrate binding region (aa 28-51), a Nudix hydrolase domain (aa 57-197) and an extended C-terminal a-helix (aa 211-219) NUDT-5 is acetylated on Met1, Lys42, Lys210, and Lys218, and phosphorylated on Tyr74. There are two isoform variants, one that shows a deletion of aa 163-165, and another that contains a 13 aa substitution for aa 164-219. Full-length human and mouse NUDT-5 share 82% aa sequence identity.

Alternate Names

Nudix motif 5, YSA1, YSA1H

Entrez Gene IDs

11164 (Human)

Gene Symbol

NUDT5

UniProt

Q9UKK9

Additional ADP-Sugar Pyrophosphatase/NUDT5 Products

Product Documents for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody

For research use only

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