Human Adrenomedullin/ADM Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB61081

R&D Systems, part of Bio-Techne
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MAB61081-100
MAB61081-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1003019

View all Adrenomedullin/ADM Antibodies »

Product Specifications

Immunogen

Human Adrenomedullin/ADM synthetic peptide
Accession # P35318

Specificity

Detects human Adrenomedullin/ADM in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Adrenomedullin/ADM Antibody

Adrenomedullin/ADM antibody in Human Kidney.

Adrenomedullin/ADM in Human Kidney.

Adrenomedullin/ADM was detected in immersion fixed sections of human kidney tissue using Mouse Anti-Human Adrenomedullin/ADM Monoclonal Antibody (Catalog # MAB61081) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and secreted molecule. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Adrenomedullin/ADM Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adrenomedullin/ADM

Adrenomedullin (ADM) is a secreted, monomeric, 6 kDa member of the adrenomedullin family of molecules.  It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes.  Adrenomedullin has multiple functions including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion.  The human Adrenomedullin preproprecursor is 185 amino acids (aa) in length.  It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-92), the Adrenomedullin precursor (amidation is required for maturation) (aa 95-147), and a C-terminal propeptide (aa 148-185).  The Adrenomedullin precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated Adrenomedullin (aa 95-146).  Depending upon the tissue, truncated forms of Adrenomedullin likely also occur, including variants spanning aa 120-146 and 128-146.  Over aa 22-147, the human Adrenomedullin proprecursor shares 70% aa identity with mouse Adrenomedullin proprecursor.       

Alternate Names

ADM

Entrez Gene IDs

133 (Human); 11535 (Mouse); 25026 (Rat)

Gene Symbol

ADM

UniProt

P35318 (Human)

Additional Adrenomedullin/ADM Products

Product Documents for Human Adrenomedullin/ADM Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Adrenomedullin/ADM Antibody

For research use only

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