Mouse IgG VisUCyte HRP Polymer Antibody

R&D Systems, part of Bio-Techne | Catalog # VC001

R&D Systems, part of Bio-Techne
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VC001-025
VC001-050
VC001-125
VC001-250
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

HRP Polymer

Antibody Source

Polyclonal Goat IgG

View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

Detects heavy ( gamma) chains on mouse IgG and light chains on all mouse immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin mouse serum proteins or serum proteins from bovine, horse, human, pig or rabbit, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IgG VisUCyte HRP Polymer Antibody

Detection of Alkaline Phosphatase in Human Liver Cancer.

Detection of Alkaline Phosphatase in Human Liver Cancer.

Alkaline Phosphatase was detected in paraffin-embedded sections of human liver cancer using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 1 µg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

Applications for Mouse IgG VisUCyte HRP Polymer Antibody

Application
Recommended Usage

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS with preservative.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Mouse IgG VisUCyte HRP Polymer Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IgG VisUCyte HRP Polymer Antibody

For research use only

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