Human AFAP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4558
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human AFAP
His450-Thr730
Accession # Q8N556
His450-Thr730
Accession # Q8N556
Specificity
Detects endogenous human AFAP in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human AFAP Antibody
Detection of Human AFAP by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human AFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4558) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AFAP at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human AFAP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line
Sample: MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AFAP
AFAP (actin filament associated protein; also known as AFAP-110) is an adaptor protein that regulates actin filament integrity. It is both multimodular and multimeric, and is known to simultaneously bind actin and cytosolic kinases. As a homomultimer, AFAP binds and separates actin filaments, preventing actin cross-linking. Upon binding to kinases, AFAP is destabilized, promoting actin polymerization. Human AFAP is 730 amino acids (aa) in length, and contains SH3 and SH2 binding motifs (aa 69‑74 and aa 91‑96), two pleckstrin homology domains (aa 153‑248 and aa 347‑450) and a Leu-zipper/actin binding region (aa 511‑637). Over aa 450‑730, human AFAP is 86% identical to mouse and rat AFAP.
Long Name
Actin Filament-associated Protein, 110 kDa
Alternate Names
AFAP-110, AFAP1
Gene Symbol
AFAP1
UniProt
Additional AFAP Products
Product Documents for Human AFAP Antibody
Product Specific Notices for Human AFAP Antibody
For research use only
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