Human AHR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6185
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 684217
Product Specifications
Immunogen
E. coli-derived recombinant human AHR
Asn704-Leu848
Accession # P35869
Asn704-Leu848
Accession # P35869
Specificity
Detects human AHR in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human AHR Antibody
AHR in Human Pancreatic Cancer Tissue.
AHR was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human AHR Monoclonal Antibody (Catalog # MAB6185) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human AHR Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AHR
AHR (Aryl-Hydrocarbon Receptor), also known as bHLHE76, is a 110 kDa member of the bHLH/PAS transcription factor family. It is widely expressed (breast, lung, liver), and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a down‑regulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing TP73 and Apaf1 genes. Human AHR is 848 amino acids (aa) in length. It contains a 10 aa prosegment, plus a 838 aa mature molecule that contains a DNA binding motif (aa 13-40), a bHLH region (aa 41-81), and two PAS domains (aa 111-342). Over aa 704-848, human AHR shares 70% aa identity with mouse AHR.
Long Name
Aryl Hydrocarbon Receptor
Alternate Names
BHLHE76
Gene Symbol
AHR
UniProt
Additional AHR Products
Product Documents for Human AHR Antibody
Product Specific Notices for Human AHR Antibody
For research use only
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