Human AIM2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9965
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Cercopithecus aethiops (African Green Monkey)
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2307A
Product Specifications
Immunogen
E. coli-derived recombinant human AIM2
Lys79-Thr180
Accession # O14862
Lys79-Thr180
Accession # O14862
Specificity
Detects human AIM2 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human AIM2 Antibody
Detection of Human AIM2 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human AIM2 Monoclonal Antibody (Catalog # MAB9965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for AIM2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human AIM2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: AIM2
Human Interferon-inducible protein AIM2 (also known as absent in melanoma 2 or simply AIM2), encoded by the AIM2 gene, is a 343 amino acids (aa), 39 KDa protein with a N-terminal DAPIN (or pyrin) domain (amino acids 1-87) and a C-terminal HIN-200 domain (amino acids 138-337), which is known to have two oligonucleotide-binding folds. Recent research has shown that AIM2 is part of the inflammasome and is involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating the inflammasome in macrophages. Upon binding to DNA, Aim-2 undergoes oligomerization and associates with PYCARD initiating the recruitment of caspase-1 precursors and processing of IL-1b and IL-18. Over aa 71-180, human Aim2 shares 90% aa sequence identity with macaque Aim2.
Long Name
Absent In Melanoma 2
Alternate Names
Gm1313, Ifi210, PYHIN4
Gene Symbol
AIM2
UniProt
Additional AIM2 Products
Product Documents for Human AIM2 Antibody
Product Specific Notices for Human AIM2 Antibody
For research use only
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