Human Akt3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1463
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 199864
Product Specifications
Immunogen
E. coli-derived recombinant human Akt3
Met1-Glu479
Accession # Q9Y243
Met1-Glu479
Accession # Q9Y243
Specificity
Detects endogenous human Akt3 in Western blots. Using Western blots, this antibody does not detect recombinant human Akt1 or Akt2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Akt3 Antibody
Detection of Human Akt3 by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human Akt3 Monoclonal Antibody (Catalog # MAB1463) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Akt3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human Akt3 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: DU145 human prostate carcinoma cell line and MDA-MB-231 human breast cancer cell line
Sample: DU145 human prostate carcinoma cell line and MDA-MB-231 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Akt3
Akt3 (v-akt murine thymoma viral oncogene homolog 3), also known as protein kinase B gamma (PKB gamma) and RAC-gamma serine/threonine kinase (RAC-PK-gamma), is a member of the Akt family that also includes Akt1 (PKB alpha) and Akt2 (PKB beta). These highly homologous kinases are central in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.
Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 3
Alternate Names
PKB gamma, RAC-gamma
Gene Symbol
AKT3
UniProt
Additional Akt3 Products
Product Documents for Human Akt3 Antibody
Product Specific Notices for Human Akt3 Antibody
For research use only
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