Detects human ALCAM/CD166 in Western blots. Shows approximately 50% cross‑reactivity with recombinant mouse OCAM and no cross-reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human ALCAM/CD166 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Western Blot

1 µg/mL
Sample: Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL) under non-reducing conditions only

Human ALCAM/CD166 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human ALCAM/CD166 Biotinylated Antibody (Catalog # BAF656)
Standard: Recombinant Human ALCAM Fc Chimera Protein, CF (Catalog # 656-AL)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

References

Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
Aruffo, A. et al. (1997) Immunol. Today 18:498.
Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Long Name

Activated Leukocyte Cell Adhesion Molecule

Alternate Names

CD166

Entrez Gene IDs

214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)

Gene Symbol

ALCAM

UniProt

Q13740

Additional ALCAM/CD166 Products

Product Documents for Human ALCAM/CD166 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ALCAM/CD166 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ALCAM/CD166 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6561

Key Product Details

Species Reactivity

Validated:

Cited:

Applications