Human Aldo-keto Reductase 1C1/AKR1C1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6529

R&D Systems, part of Bio-Techne
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MAB6529
MAB6529-SP
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Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 859026

View all Aldo-keto Reductase 1C1/AKR1C1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Aldo‑keto Reductase 1C1/AKR1C1
Met1-Tyr323
Accession # Q04828

Specificity

Detects human Aldo‑keto Reductase 1C1/AKR1C1 in ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Aldo-keto Reductase 1C3 and 1C4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Aldo-keto Reductase 1C1/AKR1C1 Antibody

Detection of Human Aldo-keto Reductase 1C1/AKR1C1 antibody by Western Blot.

Detection of Human Aldo‑keto Reductase 1C1/AKR1C1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aldo-keto Reductase 1C1/AKR1C1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Aldo-keto Reductase 1C1/AKR1C1 antibody by Simple WesternTM.

Detection of Human Aldo‑keto Reductase 1C1/AKR1C1 by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for Aldo‑keto Reductase 1C1/AKR1C1 at approximately 41 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Aldo‑keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Aldo-keto Reductase 1C1/AKR1C1 Antibody

Application
Recommended Usage

Simple Western

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and human liver tissue

Western Blot

0.1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and human liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aldo-keto Reductase 1C1/AKR1C1

AKR1C1 (20-alpha -hydroxysteroid dehydrogenase, 20‑ alpha‑HSD) is a member of aldo-keto reductase (AKR) superfamily. AKRs perform the NAD(P)H-dependent reduction of carbonyl groups (1). Four AKR1C isoforms (AKR1C1-C4) are known to exist in humans. They are all highly expressed in the liver. Three isoforms, excluding AKR1C4, have a wider expression pattern including prostate, testes, uterus, mammary gland, and haemopoietic progenitors (2). These enzymes are able to accept various natural steroids as substrates, including 3-, 7-, and 20‑ketosteroids (3). They can also activate prodrugs such as synthetic steroid hormone tibolone by converting it into active 3 alpha/ beta-hydroxy form (4). They are recognized as phase I drug-metabolizing enzymes involved in the maintenance of steroid homeostasis, prostaglandin metabolism, and metabolic activation of polycyclic aromatic hydrocarbons (5). Their reactions introduce a hydroxyl group into the product making it available for sulfonation and glucuronidation by phase II enzyme. Elevated expression of these enzymes is related to cancer with hormone-dependent malignancies (6, 7). Increased levels of expression of AKR1C1 parallels increased cell proliferation activity in human colon cancer cells. It has been shown to be associated with oncogenic potential and proproliferative effects. It is also involved in cancer cell chemoresistance.

References

  1. Jez, J.M. et al. (1997) Biochem J. 326:499.
  2. Penning, T.M. et al. (2000) Biochem. J. 351:67.
  3. Rizner, T.L. et al. (2003) Endocrinology. 144:2922.
  4. Steckelbroeck, S. et al. (2006) J. Pharmacol. Exp. Ther. 316:1300.
  5. Penning, T.M. et al. (2004) Mol. Cell. Endocrinol. 1784:1342.
  6. Penning, T.M. and M.C. Byrns (2009) Ann. N. Y. Acad. Sci. 1155:33.
  7. Baumann, D.R. et al. (2004) Drug News Perspect. 17:563.

Long Name

Aldo-keto Reductase Family 1, Member C1

Alternate Names

2-ALPHA-HSD, 20-ALPHA-HSD, AKR1C1, AldoketoReductase 1C1, DDH1, HAKRC, HBAB

Entrez Gene IDs

1645 (Human)

Gene Symbol

AKR1C1

UniProt

Q04828

Additional Aldo-keto Reductase 1C1/AKR1C1 Products

Product Documents for Human Aldo-keto Reductase 1C1/AKR1C1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Aldo-keto Reductase 1C1/AKR1C1 Antibody

For research use only

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