Human ALK-7 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7749
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ALK‑7
Met1-Glu113
Accession # Q8NER5
Met1-Glu113
Accession # Q8NER5
Specificity
Detects human ALK‑7 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ALK-7 Antibody
ALK‑7 in Human Breast Cancer Tissue.
ALK-7 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human ALK-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7749) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human ALK-7 Antibody
Application
Recommended Usage
Immunohistochemistry
10-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ALK-7
Long Name
Activin Receptor-like Kinase 7
Alternate Names
Activin RIC, ACVR1C, ACVRLK7, ALK7
Gene Symbol
ACVR1C
UniProt
Additional ALK-7 Products
Product Documents for Human ALK-7 Antibody
Product Specific Notices for Human ALK-7 Antibody
For research use only
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