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Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1448A

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # B4-78

Product Specifications

Immunogen

Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL

Specificity

Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

Detection of Alkaline Phosphatase/ALPL antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL APC-conjugated Monoclonal Antibody (Catalog # FAB1448A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Alkaline Phosphatase/ALPL in iPSCs by Flow Cytometry

iPSCs were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL APC-conjugated Monoclonal Antibody (Catalog # FAB1448A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: BG01V human embryonic stem cells; iPSCs
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using FAB1448A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a Glycosyl Phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References

  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Long Name

Alkaline Phosphatase Liver

Alternate Names

Akp2, AP-TNAP, HOPS, TNAP, TNSALP

Entrez Gene IDs

249 (Human); 11647 (Mouse)

Gene Symbol

ALPL

Additional Alkaline Phosphatase/ALPL Products

Product Documents for Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

For research use only

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