Human alpha 1B-Glycoprotein Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7757
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 875431
Product Summary for Human alpha 1B-Glycoprotein Antibody
Immunogen
HEK293 human embryonic kidney cell line transfected with human alpha 1B-Glycoprotein
Ala22-Ser495
Accession # P04217
Ala22-Ser495
Accession # P04217
Specificity
Detects human alpha 1B-Glycoprotein in ELISAs and Western blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human alpha 1B-Glycoprotein Antibody
Detection of Human alpha 1B-Glycoprotein by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a 1B-Glycoprotein Monoclonal Antibody (Catalog # MAB7757) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a 1B-Glycoprotein at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human alpha 1B-Glycoprotein Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human plasma
Sample: Human plasma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1B-Glycoprotein
Alpha-1 B-glycoprotein (A1BG), also known as C44 (in rodent) is a 68-80 kDa monomeric plasma glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed by multiple cell types, principally hepatocytes in response to circulating growth hormone. Initially thought to act as an MMP inhibitor, A1BG is now known to bind to CRISP3, a distant member of a family of venomous molecules. CRISP3 is widely expressed and possesses no toxic properties. But it may play a key role in fertilization, where it temporarily blocks the reaction of PMNs to sperm in the uterus, thus increasing sperm lifespan and the likelihood of fertilization. Within this context, A1BG may regulate CRISP3 availability. A1BG has also been investigated as a biomarker in various cancers. Mature human A1BG is 474 amino acids (aa) in length (aa 22-495) and contains five V-type Ig-like domains. There is one isoform variant that utilizes Met123 as an alternative start site. Full-length human A1BG (aa 22-495) shares only 44% aa sequence identity with mouse A1BG.
Alternate Names
A1B, A1BG, ABG, alpha 1BGlycoprotein, GAB
Gene Symbol
A1BG
UniProt
Additional alpha 1B-Glycoprotein Products
Product Documents for Human alpha 1B-Glycoprotein Antibody
Product Specific Notices for Human alpha 1B-Glycoprotein Antibody
For research use only
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