Human alpha2-Macroglobulin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB19381
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 257303
Product Specifications
Immunogen
Human plasma-derived alpha2-Macroglobulin
Specificity
Detects human alpha2-Macroglobulin in direct ELISA, this antibody does not cross-react with rhC5a or rmC5a.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human alpha2-Macroglobulin Antibody
Human alpha2-Macroglobulin ELISA Standard Curve.
Recombinant Human alpha2-Macroglobulin protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha2-Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha2-Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human alpha2-Macroglobulin Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human alpha2‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha 2-Macroglobulin DuoSet ELISA Kit (Catalog # DY1938) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2-Macroglobulin
Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.
Additional alpha 2-Macroglobulin Products
Product Documents for Human alpha2-Macroglobulin Antibody
Product Specific Notices for Human alpha2-Macroglobulin Antibody
For research use only
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