Human alpha2-Macroglobulin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1938
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Canine
Applications
Validated:
Simple Western, Western Blot
Cited:
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 257316
Product Specifications
Immunogen
Human plasma-derived alpha2-Macroglobulin
Specificity
Detects human alpha2-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with rhC5a or rmC5a.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human alpha2-Macroglobulin Antibody
Detection of Human alpha2‑Macroglobulin by Western Blot.
Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human alpha2‑Macroglobulin by Simple WesternTM.
Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human alpha2-Macroglobulin Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Human serum
Sample: Human serum
Western Blot
2 µg/mL
Sample: Human serum
Sample: Human serum
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 3.7 using MAB1938 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2-Macroglobulin
Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.
Additional alpha 2-Macroglobulin Products
Product Documents for Human alpha2-Macroglobulin Antibody
Product Specific Notices for Human alpha2-Macroglobulin Antibody
For research use only
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