Human alpha-Aminoadipate Aminotransferase Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7927
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human alpha-Aminoadipate Aminotransferase
Met1-Leu425
Accession # Q8N5Z0
Met1-Leu425
Accession # Q8N5Z0
Specificity
Detects human alpha-Aminoadipate Aminotransferase in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human alpha-Aminoadipate Aminotransferase Antibody
Detection of Human alpha-Aminoadipate Aminotransferase by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Aminoadipate Aminotransferase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7927) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Aminoadipate Aminotransferase at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human alpha-Aminoadipate Aminotransferase Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Aminoadipate Aminotransferase
Alpha-Aminoadipate Aminotransferase (AADAT, KAT2 and Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial) is a 47 kDa (predicted) mitochondrial member of the class-I pyridoxal-phosphate-dependent aminotransferase family of proteins. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. Human ADDAT is predominantly expressed in the liver, it is also found in heart, brain, kidney, pancreas, prostate, testis and ovary. Over aa 1-425, human AADAT shares 73% aa sequence identity with mouse ADDAT.
Long Name
alpha-Aminoadipate Aminotransferase
Alternate Names
AADAT, alphaAminoadipate Aminotransferase, KAT2
Gene Symbol
AADAT
UniProt
Additional alpha-Aminoadipate Aminotransferase Products
Product Documents for Human alpha-Aminoadipate Aminotransferase Antibody
Product Specific Notices for Human alpha-Aminoadipate Aminotransferase Antibody
For research use only
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