Human alpha-Parvin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5556
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human alpha-Parvin
Val46-Phe133
Accession # Q9NVD7
Val46-Phe133
Accession # Q9NVD7
Specificity
Detects human alpha-Parvin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human beta-Parvin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human alpha-Parvin Antibody
Detection of Human alpha-Parvin by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Parvin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Parvin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human alpha-Parvin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and human tonsil tissue
Sample: HUVEC human umbilical vein endothelial cells and human tonsil tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Parvin
alpha-Parvin (parvus [Latin for "small"]-A/ alpha; also actopaxin and PARV-A) is a 53 kDa member of the parvin family, alpha‑actinin superfamily of proteins. It is widely expressed, and participates in actin filament assembly that precedes cell migration. Human PARVA is 372 amino acids (aa) in length. It contains two CH/calponin homology domains (aa 99‑202 and 265‑369), two NLSs (aa 19‑26 and 38‑40), and a recently identified paxillin binding sequence (aa 248‑256). Multiple phosphorylation sites occur between Ser4 and Thr16 that are apparently utilized. PARVA is likely to exist as a dimer. There are three isoform variants of PARVA. All three contain an alternate start site at Met41, with one also showing a 21 aa substitution for aa 239‑372, and a second also showing a 46 aa substitution for aa 134‑372, respectively. Over aa 46‑133, human and mouse PARVA share 95% aa identity.
Alternate Names
Actopaxin, alphaParvin, CH-ILKBP, MXRA2, PARVA
Gene Symbol
PARVA
UniProt
Additional alpha-Parvin Products
Product Documents for Human alpha-Parvin Antibody
Product Specific Notices for Human alpha-Parvin Antibody
For research use only
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