Human alpha-Sarcoglycan Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7445

R&D Systems, part of Bio-Techne
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MAB7445-100
MAB7445-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 769109

View all alpha-Sarcoglycan Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human alpha-Sarcoglycan
Gln24-Ala290 (predicted)
Accession # Q16586

Specificity

Detects human alpha-Sarcoglycan in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human alpha-Sarcoglycan Antibody

Detection of Human a-Sarcoglycan antibody by Western Blot.

Detection of Human alpha-Sarcoglycan by Western Blot.

Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human a-Sarcoglycan Monoclonal Antibody (Catalog # MAB7445) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Sarcoglycan at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human alpha-Sarcoglycan Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human skeletal muscle tissue and human heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Sarcoglycan

Alpha-sarcoglycan is one of six known sarcoglycans, which are type I ( alpha- and epsilon-) or type II ( beta-, gamma-, delta- or zeta-) transmembrane glycoproteins. Sarcoglycans alpha and gamma are expressed exclusively in the sarcoplasmic reticulum in skeletal and cardiac muscle, while others are more widely expressed. Sarcoglycans form heterotetrameric, pentameric and hexameric membrane glycoprotein complexes (SGC). SGC interacts laterally with the dystroglycan complex, and both complexes are components of the dystrophin-associated glycoprotein complex (DGC), which forms a link between the cytoskeleton and the extracellular matrix. Mutations in sarcoglycans are associated with recessive autosomally inherited limb-girdle muscular dystrophy. Human alpha-sarcoglycan shares 90% amino acid sequence identity with mouse and rat alpha-sarcoglycan within the extracellular domain.

Alternate Names

50-DAG, Adhalin, ADL, alphaSarcoglycan, DAG2, DMDA2, Dystroglycan-2, LGMD2D, SCARMD1, SGCA

Entrez Gene IDs

6442 (Human); 20391 (Mouse); 303468 (Rat)

Gene Symbol

SGCA

UniProt

Q16586

Additional alpha-Sarcoglycan Products

Product Documents for Human alpha-Sarcoglycan Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human alpha-Sarcoglycan Antibody

For research use only

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