Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin PE-conjugated Monoclonal Antibody (Catalog # IC1420P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human alpha-Smooth Muscle Actin PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References

Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
Oishi, K. et al. (2002) J. Physiol. 540:139.

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Entrez Gene IDs

59 (Human); 11475 (Mouse); 81633 (Rat)

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for Human alpha-Smooth Muscle Actin PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human alpha-Smooth Muscle Actin PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human alpha-Smooth Muscle Actin PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1420P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all alpha-Smooth Muscle Actin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human alpha-Smooth Muscle Actin PE-conjugated Antibody

Detection of alpha‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry.