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Human alpha-Synuclein Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB13384

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB13384-100
MAB13384-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 913630R

Product Specifications

Immunogen

E. coli-derived recombinant human alpha-Synuclein
Met1-Ala140
Accession # P37840

Specificity

Detects human alpha-Synuclein in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human alpha-Synuclein Antibody

Human a-Synuclein Antibody in ELISA Standard Curve.

Human alpha-Synuclein ELISA Standard Curve.

Recombinant Human a-Synuclein protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Synuclein Monoclonal Antibody (Catalog # MAB13384) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human a-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human alpha-Synuclein Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human alpha-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha-Synuclein DuoSet ELISA Kit (Catalog # DY1338-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Synuclein

alpha-Synuclein (SNCA) is a cytoplasmic protein that is predominantly expressed in the central nervous system. It is a minor protein found in amyloid deposition that is a hallmark of Alzheimer's disease. Defects in SNCA are associated with familial Parkinson's disease.

Alternate Names

NACP, PARK1, PARK4, SNCA, Synuclein-alpha

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Gene Symbol

SNCA

UniProt

Additional alpha-Synuclein Products

Product Documents for Human alpha-Synuclein Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human alpha-Synuclein Antibody

For research use only

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