Human alpha1-Acid Glycoprotein Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3694
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 386131
Product Specifications
Immunogen
Human plasma-derived alpha1-Acid Glycoprotein
Accession # P02763
Accession # P02763
Specificity
Detects human alpha1‑Acid Glycoprotein in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human alpha1-Acid Glycoprotein Antibody
Detection of Human alpha1‑Acid Glycoprotein by Western Blot.
Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for a1-Acid Glycoprotein at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human alpha1‑Acid Glycoprotein by Western Blot.
Western blot shows lysates of human plasma. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a1-Acid Glycoprotein at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha1-Acid Glycoprotein in Human Liver.
a1-Acid Glycoprotein was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human alpha1-Acid Glycoprotein Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Western Blot
2 µg/mL
Sample: Human liver tissue and Human plasma
Sample: Human liver tissue and Human plasma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3694 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Acid Glycoprotein
alpha1-Acid Glycoprotein (AGP-1), also known as orosomucoid-1, is an acute phase protein secreted by the liver. Serum AGP-1 levels are elevated during inflammatory responses. AGP-1 binds a wide range of molecules in the circulation. Human and mouse AGP-1 share 43% amino acid sequence identity.
Alternate Names
AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid
Gene Symbol
ORM1
UniProt
Additional alpha 1-Acid Glycoprotein Products
Product Documents for Human alpha1-Acid Glycoprotein Antibody
Product Specific Notices for Human alpha1-Acid Glycoprotein Antibody
For research use only
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