Human Aminopeptidase LRAP/ERAP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3830
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase LRAP/ERAP2
Ala56-Thr960
Accession # Q6P179
Ala56-Thr960
Accession # Q6P179
Specificity
Detects human Aminopeptidase LRAP/ERAP2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Aminopeptidase LRAP/ERAP2 Antibody
Detection of Human Aminopeptidase LRAP/ERAP2 by Western Blot
Immunoblot analyses of ERAP2 using LCL protein extract.Two LCLs of each ERAP2 genotype (AA, AB, and BB) were tested for protein using primary antibodies specific to: A, ERAP2 (goat polyclonal); B, ERAP2 (mouse polyclonal); and C, beta-actin (see Materials and Methods). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1001157), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Aminopeptidase LRAP/ERAP2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN)
Sample: Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF3830 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase LRAP/ERAP2
Leukocyte-derived arginine aminopeptidase (LRAP) and endoplasmic reticulum-aminopeptidase 2 (ERAP2) are the two names given to the protein encoded by the LRAP gene (1, 2). Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors. It belongs to the oxytocinase subfamily of M1 aminopeptidases, which also includes aminopeptidases PILS/ARTS1/ERAP1 and LNPEP/PLAP (1, 3). In addition to antigen presentation, the members of this subfamily are also important in maintenance of pregnancy, memory retention, and blood pressure regulation (3).
References
- Tanioka, T. et al. (2003) J. Biol. Chem. 278:32275.
- Tanioka, T. et al. (2005) FEBS J. 272:916.
- Tsujimoto, M. and A. Hattori (2005) Biochim. Biophys. Acta 1751:9.
Long Name
Leukocyte-derived Arginine Aminopeptidase
Alternate Names
ERAP2
Entrez Gene IDs
64167 (Human)
Gene Symbol
ERAP2
UniProt
Additional Aminopeptidase LRAP/ERAP2 Products
Product Documents for Human Aminopeptidase LRAP/ERAP2 Antibody
Product Specific Notices for Human Aminopeptidase LRAP/ERAP2 Antibody
For research use only
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