Human Aminopeptidase PILS/ARTS1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB23341
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 325813
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase PILS/ARTS1
Thr25-Ile929
Accession # Q9NZ08
Thr25-Ile929
Accession # Q9NZ08
Specificity
Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Aminopeptidase PILS/ARTS1 Antibody
Aminopeptidase PILS/ARTS1 in HeLa Human Cell Line.
Aminopeptidase PILS/ARTS1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB23341) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Aminopeptidase PILS/ARTS1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase PILS/ARTS1
Aminopeptidase PILS/ARTS1 is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum). ARTS1 is widely expressed and releases an N-terminal amino acid (Leu). The amino acid sequence of mouse ARTS1 is 94%, 84%, 80 and 70% identical to that of rat, human, canine, and chicken.
Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Alternate Names
A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP
Gene Symbol
ERAP1
UniProt
Additional Aminopeptidase PILS/ARTS1 Products
Product Documents for Human Aminopeptidase PILS/ARTS1 Antibody
Product Specific Notices for Human Aminopeptidase PILS/ARTS1 Antibody
For research use only
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