Human Aminopeptidase PILS/ARTS1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2334
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase PILS/ARTS1
Ala37-Met941
Accession # EAW96077
Ala37-Met941
Accession # EAW96077
Specificity
Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross‑reactivity with recombinant mouse ARTS1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Aminopeptidase PILS/ARTS1 Antibody
Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Aminopeptidase PILS/ARTS1 in A549 Human Cell Line.
Aminopeptidase PILS/ARTS1 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Aminopeptidase PILS/ARTS1 in Human Liver.
Aminopeptidase PILS/ARTS1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Aminopeptidase PILS/ARTS1 Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase PILS/ARTS1 (Catalog # 2334-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase PILS/ARTS1 (Catalog # 2334-ZN), see our available Western blot detection antibodies
Simple Western
10 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cells and HDLM‑2 human Hodgkin's lymphoma cells
Sample: COLO 205 human colorectal adenocarcinoma cells and HDLM‑2 human Hodgkin's lymphoma cells
Western Blot
1 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line and HDLM‑2 human Hodgkin's lymphoma cell line
Sample: COLO 205 human colorectal adenocarcinoma cell line and HDLM‑2 human Hodgkin's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase PILS/ARTS1
The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro (1). Also known as ALAP (Adipocyte-derived Leucin AiminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2‑4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2‑5).
References
- Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego.
- Cui, X. et al. (2002) J. Clin. Invest. 110:515.
- York, I.A. et al. (2002) Nat. Immunol. 3:1177.
- Serwold, T. et al. (2002) Nature 419:480.
- Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.
Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Alternate Names
A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP
Gene Symbol
ERAP1
UniProt
Additional Aminopeptidase PILS/ARTS1 Products
Product Documents for Human Aminopeptidase PILS/ARTS1 Antibody
Product Specific Notices for Human Aminopeptidase PILS/ARTS1 Antibody
For research use only
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