Human AMPK beta2 Antibody

Name: Human AMPK beta2 Antibody
Brand: R&D Systems
SKU: MAB11639

R&D Systems, part of Bio-Techne | Catalog # MAB11639

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 1092615

View all AMPK beta 2 Antibodies »

Product Specifications

Immunogen

E. coli- derived recombinant human AMPKbeta2
Met1-Ile272
Accession # O43741

Specificity

Detects recombinant human AMPK beta2 protein in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human AMPK beta2 Antibody

Detection of Human AMPK beta2 by Western Blot.

Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human AMPK beta2 Monoclonal Antibody (Catalog # MAB11639) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPK beta2 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human AMPK beta2 by Simple Western^TM.

Simple Western shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.5 mg/ml. A specific band was detected for AMPK beta2 at approximately 38 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human AMPK beta2 Monoclonal Antibody (Catalog # MAB11639). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human AMPK beta2 Antibody

Application

Recommended Usage

Simple Western

20 µg/mL
Sample: HEK293T human embryonic kidney cell line

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line and U2OS human osteosarcoma

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMPK beta 2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3), with all 12 combinations able to form complexes. The beta1 subunit is expressed at higher levels than the beta2subunit in liver, while beta2 is more abundant in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase beta 2

Alternate Names

PRKAB2

Entrez Gene IDs

5565 (Human); 108097 (Mouse); 64562 (Rat)

Gene Symbol