Human Angiopoietin-like Protein 6/ANGPTL6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB113061
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 856630
Product Specifications
Immunogen
E. coli-expressed recombinant Human Angiopoietin-like Protein 6/ANGPTL6
Arg21-Leu470
Accession # Q8ROZ6
Arg21-Leu470
Accession # Q8ROZ6
Specificity
Detects human Angiopoietin-like protein 6/ANGPTL6 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Angiopoietin-like Protein 6/ANGPTL6 Antibody
Human Angiopoietin-like Protein 6/ANGPTL6 ELISA Standard Curve.
Recombinant Human Angiopoietin-like Protein 6/ANGPTL6 protein was serially diluted 2-fold and captured by Mouse Anti-Human Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (MAB11306) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB113061) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Angiopoietin-like Protein 6/ANGPTL6 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with
Human Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB11306). This product is
intended for assay development on various assay platforms requiring
antibody pairs.
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Angiopoietin-like 6 DuoSet ELISA Kit (Catalog # DY7846-05) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 6/ANGPTL6
126-164) and a fibrinogen-like domain (aa 238-456). A conserved Integrin-binding RGD motif is located within the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and promotes insulin sensitivity and energy expenditure in mice fed high fat diets. Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and preeclampsia but are decreased in chronic renal failure. ANGPTL6 is additionally produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.
Alternate Names
AGF, Angiopoietin like Protein 6, ANGPTL6, ARP5
Gene Symbol
ANGPTL6
UniProt
Additional Angiopoietin-like Protein 6/ANGPTL6 Products
Product Documents for Human Angiopoietin-like Protein 6/ANGPTL6 Antibody
Product Specific Notices for Human Angiopoietin-like Protein 6/ANGPTL6 Antibody
For research use only
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