Human Angiostatin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF226
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Parasite - Plasmodium falciparum
Applications
Validated:
Western Blot
Cited:
ELISA Development, Immunocytochemistry, Immunogold Electron Microscopy, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Angiostatin Kringle 1-3
Val98-Ser358 with Cys188Ser & Cys316Ser substitutions
Accession # P00747
Val98-Ser358 with Cys188Ser & Cys316Ser substitutions
Accession # P00747
Specificity
Detects human Angiostatin in direct ELISAs and Western blots. This antibody will also recognize human plasminogen.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Angiostatin Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Angiostatin Kringle 1-3
Sample: Recombinant Human Angiostatin Kringle 1-3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiostatin
Angiostatin is a 38-45 kDa proteolytic fragment of plasminogen, a 92-100 kDa glycosylated blood zymogen that serves as the precursor for plasmin. Mature human plasminogen is 713 amino acids (aa) in length (aa 98-810). It contains five N-terminal kringle domains (aa 103-561) plus one peptidase S1 domain (aa 581-808). Upon cleavage by tPA between Arg580 and Val581, plasminogen is converted into the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots. Plasminogen is also cleaved by MMPs which generate multiple fragments. Angiostatin has documented antiangiogenic and antitumor activity, likely mediated by a downregulation of Bcl2, and an upregulation of thrombospondin-1. Over aa 98-358, human angiostatin shares 81% aa identity with mouse angiostatin.
Additional Angiostatin Products
Product Documents for Human Angiostatin Antibody
Product Specific Notices for Human Angiostatin Antibody
For research use only
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