Human APAF-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB868
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat, Avian - Chicken
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 94408
Product Specifications
Immunogen
E. coli-derived recombinant human APAF-1
Leu10-Asp214
Accession # AAC51678
Leu10-Asp214
Accession # AAC51678
Specificity
Detects human APAF-1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human APAF-1 Antibody
Detection of Human APAF-1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105cells (lane 1) and 7 x 104cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF-1 Monoclonal Antibody (Catalog # MAB868) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for APAF-1 was detected at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Human APAF-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APAF-1
Apoptosis protease-activating factor 1 (APAF‑1) is a ubiquitous 135 kDa protein component of the cytoplasmic apoptosome complex. APAF‑1 plays an important role in the mitochondrial apoptotic pathway by triggering the Cytochrome c-dependent activation of Caspase-9. APAF‑1 participates in tissue morphogenesis during development, and loss of APAF‑1 expression is associated with tumor progression in a variety of cancers. Some alternate splice forms of APAF‑1 are deficient in their ability to promote apoptosis.
Long Name
Apoptotic Peptidase Activating Factor 1
Alternate Names
APAF1
Entrez Gene IDs
317 (Human)
Gene Symbol
APAF1
UniProt
Additional APAF-1 Products
Product Documents for Human APAF-1 Antibody
Product Specific Notices for Human APAF-1 Antibody
For research use only
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