Human APJ Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8561
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 72133R
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human APJ
Met1-Asp380
Accession # P35414
Met1-Asp380
Accession # P35414
Specificity
Detects human APJ in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human APJ Antibody
Detection of APJ in U‑118‑MG Human Cell Line by Flow Cytometry.
U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human APJ Monoclonal Antibody (Catalog # MAB8561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
APJ in Human Hypothalamus.
APJ was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Mouse Anti-Human APJ Monoclonal Antibody (Catalog # MAB8561) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human APJ Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Immunohistochemistry
0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human hypothalamus
Sample: Immersion fixed paraffin-embedded sections of human hypothalamus
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APJ
APJ is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, apelin. APJ can also function as a co-receptor for HIV.
Long Name
Apelin Receptor/G-protein Coupled Receptor APJ
Alternate Names
AGTRL1, APJR, APLNR, HG11
Gene Symbol
APLNR
UniProt
Additional APJ Products
Product Documents for Human APJ Antibody
Product Specific Notices for Human APJ Antibody
For research use only
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