Detects human APLP-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse APLP‑1 is observed and no cross-reactivity with recombinant human APLP‑2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human APLP-1 Antibody

APLP-1 in SH-SY5Y Human Cell Line.

APLP-1 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line (positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line (negative staining) using Mouse Anti-Human APLP-1 Monoclonal Antibody (Catalog # MAB3129) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

APLP-1 in TT Human Cell Line.

APLP-1 was detected in immersion fixed TT human medullary thyroid cancer cell line (positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line (negative staining) using Mouse Anti-Human APLP-1 Monoclonal Antibody (Catalog # MAB3129) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human APLP-1 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line and TT human medullary thyroid cancer cell line

Reviewed Applications

Read 1 review rated 5 using MAB3129 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APLP-1

APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and shares 89% amino acid sequence identity between human and mouse.

Long Name

Amyloid-like Protein 1

Alternate Names

APLP1

Entrez Gene IDs

333 (Human); 11803 (Mouse); 502317 (Rat)

Gene Symbol

APLP1

UniProt

P51693

Additional APLP-1 Products

Product Documents for Human APLP-1 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human APLP-1 Antibody

For research use only

Citations
Reviews

Human APLP-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3129

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity