Human APLP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB49451
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Bioassay, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 397702
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human APLP-2
Leu30-Ser680
Accession # Q06481
Leu30-Ser680
Accession # Q06481
Specificity
Detects human APLP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse APLP-2 or recombinant human APLP-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human APLP-2 Antibody
Detection of Human APLP‑2 by Western Blot.
Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Human APLP-2 Monoclonal Antibody (Catalog # MAB49451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APLP-2 at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human APLP-2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human brain (Alzheimer's disease hippocampus) tissue under non-reducing conditions only
Sample: Human brain (Alzheimer's disease hippocampus) tissue under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APLP-2
Amyloid beta precursor-like protein 2 (APLP-2) is a 120-170 kDa member of the APP family of neuronal type I transmembrane proteins. Its extracellular domain consists of an N-terminal Cys-rich domain, an Asp/Glu-rich acidic region, a Kunitz protease inhibitor (KPI) domain, and a GAG attachment site in the membrane proximal domain. APLP-2 forms both homodimers and heterodimers with APP and APLP-1. Proteolytic cleavage of APLP-2 generates peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that functions as a transcriptional coactivator. Alternate splicing of APLP-2 generates isoforms that lack the KPI domain or contain an insertion that prevents GAG attachment. The extracellular domain of human APLP-2 shares 81% and 93% aa sequence identity with mouse and rat APLP-2, respectively.
Long Name
Amyloid Beta [A4] Precursor-like Protein 2
Alternate Names
APLP2, APPH, APPL2, CDEBP, WALPLP2, YWK-II
Gene Symbol
APLP2
UniProt
Additional APLP-2 Products
Product Documents for Human APLP-2 Antibody
Product Specific Notices for Human APLP-2 Antibody
For research use only
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