Human Apolipoprotein B/ApoB Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3556
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Co-Immunoprecipitation, Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Apolipoprotein B/ApoB
Met1206-Asp1413
Accession # NP_000375
Met1206-Asp1413
Accession # NP_000375
Specificity
Detects human Apolipoprotein B/ApoB in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Apolipoprotein B/ApoB Antibody
Detection of Human Apolipoprotein B/ApoB by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Apolipoprotein B/ApoB in Human Liver.
Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm and plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Apolipoprotein B/ApoB by Simple WesternTM.
Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B/ApoB at approximately 312 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.Applications for Human Apolipoprotein B/ApoB Antibody
Application
Recommended Usage
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Simple Western
5 µg/mL
Sample: Human plasma
Sample: Human plasma
Western Blot
1 µg/mL
Sample: Human plasma
Sample: Human plasma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein B/ApoB
Alternate Names
APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4
Gene Symbol
APOB
UniProt
Additional Apolipoprotein B/ApoB Products
Product Documents for Human Apolipoprotein B/ApoB Antibody
Product Specific Notices for Human Apolipoprotein B/ApoB Antibody
For research use only
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