Human Apolipoprotein M/ApoM Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4550
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ApoM
Cys23-Asn188
Accession # O95445
Cys23-Asn188
Accession # O95445
Specificity
Detects endogenous human ApoM in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Apolipoprotein M/ApoM Antibody
Detection of Human Apolipoprotein M/ApoM by Western Blot.
Western blot shows lysates of human plasma and liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein M/ApoM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4550) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein M/ApoM at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.Applications for Human Apolipoprotein M/ApoM Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Human plasma and liver tissue
Sample: Human plasma and liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein M/ApoM
Apolipoprotein M (ApoM; also known as Protein G3a) is a 25 kDa, secreted member of the lipocalin family of proteins. It is produced by hepatocytes and renal tubular epithelium, and represents a minor component of HDL particles. Human ApoM is 188 amino acids (aa) in length. It contains an uncleaved, hydrophobic N-terminal segment and one utilized N-linked glycosylation site (mouse ApoM shows no glycosylation). At least two potential isoforms exist. One shows an alternate start site at Met73, while a second shows the same start site plus a 21 aa substitution for aa 181‑188. Human ApoM shares 91% and 80% aa identity with pig and mouse ApoM, respectively.
Alternate Names
APOM, NG20, Protein G3a
Gene Symbol
APOM
UniProt
Additional Apolipoprotein M/ApoM Products
Product Documents for Human Apolipoprotein M/ApoM Antibody
Product Specific Notices for Human Apolipoprotein M/ApoM Antibody
For research use only
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