Human APRIL/TNFSF13 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5860

R&D Systems, part of Bio-Techne
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MAB5860
MAB5860-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 670820

View all APRIL/TNFSF13 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human APRIL/TNFSF13
Ala105-Leu250
Accession # O75888

Specificity

Detects human APRIL/TNFSF13 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse APRIL is observed, and no cross-reactivity with recombinant human BAFF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human APRIL/TNFSF13 Antibody

Cell Proliferation Induced by APRIL/TNFSF13 and Neutralization by Human APRIL/TNFSF13 Antibody.

Cell Proliferation Induced by APRIL/TNFSF13 and Neutralization by Human APRIL/TNFSF13 Antibody.

Recombinant Human APRIL/TNFSF13 induces proliferation in mouse B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND50 is typically 0.15-0.75 µg/mL.

Applications for Human APRIL/TNFSF13 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize APRIL/TNFSF13-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 0.15-0.75 ug/mL in the presence of 200 ng/mL Recombinant Human APRIL/TNFSF13.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APRIL/TNFSF13

APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat)

Gene Symbol

TNFSF13

UniProt

O75888 (Human)

Additional APRIL/TNFSF13 Products

Product Documents for Human APRIL/TNFSF13 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human APRIL/TNFSF13 Antibody

For research use only

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