Human B7-H7/HHLA2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8084
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived human B7-H7/HHLA2
Met1-Asn344
Accession # Q9UM44
Met1-Asn344
Accession # Q9UM44
Specificity
Detects human B7-H7/HHLA2 in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human B7-H7/HHLA2 Antibody
Detection of Human B7-H7/HHLA2 by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidny cell line transfected with human B7-H7. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H7/HHLA2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
B7-H7/HHLA2 in Human Monocytes.
B7-H7/HHLA2 was detected in immersion fixed human monocytes using Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human B7-H7/HHLA2 Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed human monocytes
Sample: Immersion fixed human monocytes
Western Blot
1 µg/mL
Sample: HEK293T human embryonic kidny cell line transfected with human B7-H7
Sample: HEK293T human embryonic kidny cell line transfected with human B7-H7
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-H7/HHLA2
B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).
References
- Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Mager, D.L. et al. (1999) Genomics 59:255.
- Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
- Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
Long Name
B7 Homolog 7
Alternate Names
B7H7, HHLA2
Gene Symbol
HHLA2
UniProt
Additional B7-H7/HHLA2 Products
Product Documents for Human B7-H7/HHLA2 Antibody
Product Specific Notices for Human B7-H7/HHLA2 Antibody
For research use only
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