Human Bag-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB852
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GP3.10G3E2
Product Specifications
Immunogen
E. coli-derived recombinant human Bag-1
Specificity
Detects the 36 kDa and 50 kDa isoforms of human Bag-1. Does not detect mouse Bag-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Bag-1 Antibody
Detection of Human Bag-1 by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Bag-1 at approximately 36 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Immunoprecipitation of Human Bag‑1.
Bag-1 was immunoprecipitated from lysates (5 x 106cells) of THP-1 human acute monocytic leukemia and HEK293 human embryonic kidney cell line following incubation with 3 µg Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) for 1-16 hours at 4 °C. Bag-1-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bag-1 (left panel) was detected by Western blot using 0.5 µg/mL Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852). View our recommended buffer recipes for immunoprecipitation.
Western Blot Shows Human Bag‑1 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bag-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Bag-1 at approximately 29, 39, and 50 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Bag-1 Antibody
Application
Recommended Usage
Immunoprecipitation
0.6 µg/106 cells
Sample: THP-1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line, see our available Western blot detection antibodies
Sample: THP-1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line, see our available Western blot detection antibodies
Knockout Validated
Bag‑1
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
Bag‑1 knockout HeLa cell line.
Western Blot
0.5 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line
Sample: THP‑1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bag-1
Bag-1 (Bcl-2-associated Athanogene 1)
is a 31-kDa protein with an apparent molecular mass of 46 kDa that can bind to
Bcl-2 and enhance its anti-apoptotic effect, resulting in increased protection
from cell death induced by apoptotic stimuli. Bag-1 shares no significant
homology with Bcl-2 or other Bcl-2 family proteins. The C-terminal region of
Bag-1 binds the PDGF and HGF Receptor, and enhances growth factor-mediated
protection from apoptosis. This represents a link between growth factor
receptors and anti-apoptotic mechanisms.
Long Name
Bcl-2-associated Anthanogene-1
Alternate Names
Bag1
Gene Symbol
BAG1
Additional Bag-1 Products
Product Documents for Human Bag-1 Antibody
Product Specific Notices for Human Bag-1 Antibody
For research use only
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