HEK293 human embryonic kidney cell line transfected with (A) human BAI1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human BAI1 Monoclonal Antibody (Catalog # MAB49692) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

BAI1 in U2OS Human Cell Line.

BAI1 was detected in immersion fixed U2OS human osteosarcoma cell line (positive stain) and MCF-7 human breast cancer cell line (negative stain) using Mouse Anti-Human BAI1 Monoclonal Antibody (Catalog # MAB49692) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human BAI1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human BAI1 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line (positive stain) and MCF‑7 human breast cancer cell line (negative stain)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAI1

Brain Angiogenesis Inhibitor 1 (BAI1) is a 170 kDa 7-transmembrane domain G protein-coupled receptor (GPCR) that has a large N-terminal extracellular region with an RGD motif, five thrombospondin type I repeats, and a juxtamembrane GPS (GPCR proteolytic cleavage site) (1). Within the extracellular domain (ECD) up to the GPS (amino acids 31‑879), mature human BAI1 shares 94% amino acid sequence identity with mouse and rat BAI1. BAI1 is preferentially expressed on brain neurons but also is found on astrocytes and macrophages and in the pancreas, stomach, and colon (1‑8). BAI1 can be cleaved within the GPS to release a 120 kDa fragment termed Vasculostatin which corresponds to nearly the entire N-terminal ECD (9). Generation of additional soluble fragments suggests the cleavage of BAI1 at multiple sites (9, 10). BAI1 fragments interact with Integrin alphaV beta5 or CD36 on microvascular endothelial cells to inhibit cell proliferation and migration (10, 11). Overexpression of BAI1 in glioblastoma or pancreatic adenocarcinoma cells inhibits their tumorigenicity and the development of tumor-associated neovascularization (6, 12). Fragments of the ECD, including Vasculostatin, also suppress in vivo angiogenesis and tumor growth (1, 9, 11). BAI1 is down‑regulated in glioblastoma, carcinomas of the pancreas, colon, and stomach and also in experimental ischemia (2, 4, 6‑8). Its expression is inversely correlated with tumor vascularity in colorectal and pulmonary carcinomas (8, 13). On macrophages and astrocytes, BAI1 mediates the phagocytosis of apoptotic cells through recognition of cell surface phosphatidylserine (5).

References

Nishimori, H. et al. (1997) Oncogene 15:2145.
Koh, J.T. et al. (2001) Brain Res. Mol. Brain Res. 87:223.
Mori, K. et al. (2002) Neurosci. Res. 43:69.
Kaur, B. et al. (2003) Am. J. Pathol. 162:19.
Park, D. et al. (2007) Nature 450:430.
Duda, D.G. et al. (2002) Br. J. Cancer 86:490.
Lee, J.H. et al. (2001) Int. J. Oncol. 18:355.
Fukushima, Y. et al. (1998) Int. J. Oncol. 13:967.
Kaur, B. et al. (2005) Oncogene 24:3632.
Koh, J.T. et al. (2004) Exp. Cell Res. 294:172.
Kaur, B. et al. (2009) Cancer Res. 69:1212.
Kang, X. et al. (2006) Cancer Gene Ther. 13:385.
Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5:181.

Long Name

Brain-specific Angiogenesis Inhibitor 1

Alternate Names

brain-specific angiogenesis inhibitor 1, FLJ41988, GDAIF

Entrez Gene IDs

575 (Human); 107831 (Mouse); 362931 (Rat)

Gene Symbol

ADGRB1

UniProt

O14514

Additional BAI1 Products

Product Documents for Human BAI1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human BAI1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human BAI1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB49692

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all BAI1 Antibodies »

Product Summary for Human BAI1 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human BAI1 Antibody

Detection of BAI1 in HEK293 Human Cell Line Transfected with Human BAI1 and eGFP by Flow Cytometry