HEK293 human embryonic kidney cell line transfected with either (A) human BAI3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human BAI3 Monoclonal Antibody (Catalog # MAB39651) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Detection of BAI3 in SHSY-5Y Human Neuroblastoma Cell Line

SHSY-5Y human neuroblastoma cell line was stained with Mouse Anti-Human BAI3 Monoclonal Antibody (Catalog # MAB39651, filled histogram) or Mouse IgG1 Isotype Control Antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human BAI3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human BAI3 and eGFP and SHSY-5Y Human Neuroblastoma Cell Line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAI3

Human BAI3 (brain-specific angiogenesis inhibitor 3) is a 177 kDa, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25‑883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296‑508), and one GPS domain (aa 816‑867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643‑665. Over aa 25‑880, human BAI3 shares 98% aa identity with mouse BAI3.

Long Name

Brain-specific Angiogenesis Inhibitor 3

Alternate Names

brain-specific angiogenesis inhibitor 3, dJ91B17.1 (brain-specific angiogenesis inhibitor 3), KIAA0550MGC133100

Entrez Gene IDs

577 (Human); 210933 (Mouse); 301309 (Rat)

Gene Symbol

ADGRB3

UniProt

O60242

Additional BAI3 Products

Product Documents for Human BAI3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human BAI3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human BAI3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB39651

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all BAI3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human BAI3 Antibody

Detection of BAI3 in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP by Flow Cytometry