Human Bax Minus C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # AB820
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Human Bax synthetic peptide
Specificity
Detects human Bax Minus C-Terminus. Does not detect mouse Bax.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Bax Minus C-Terminus Antibody
Detection of Human Bax Minus C-Terminus by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 4 µg/mL Goat Anti-Human Bax Minus C-Terminus Polyclonal Antibody (Catalog # AB820) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human Bax (1 ng) was included. A specific band for Bax was detected at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human Bax Minus C-Terminus Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast
Sample: Immersion fixed paraffin-embedded sections of human breast
Western Blot
4 µg/mL
Sample: THP-1 human acute monocytic leukemia cell line
Sample: THP-1 human acute monocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bax
Long Name
Bcl Associated X Protein
Alternate Names
apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4
Gene Symbol
BAX
UniProt
Additional Bax Products
Product Documents for Human Bax Minus C-Terminus Antibody
Product Specific Notices for Human Bax Minus C-Terminus Antibody
For research use only
Loading...
Loading...
Loading...
Loading...