Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta₂-Microglobulin Monoclonal Antibody (Catalog # MAB82481) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta₂-Microglobulin at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Beta 2 Microglobulin in Human PBMC lymphocytes by Flow Cytometry.

Human PBMC lymphocytes were stained with Mouse Anti-Human Beta 2 Microglobulin Monoclonal Antibody (Catalog # MAB82481, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human beta₂-Microglobulin Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human PBMC lymphocytes

Western Blot

2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta 2-Microglobulin

beta₂-Microglobulin (b2M) is a 12 kDa secreted polypeptide that serves as the light chain of Class I MHC molecules. Possessing an Ig-like domain, b2M noncovalently associates with both 44 kDa classical (HLA-A, -B, -C) and 40 kDa non-classical (HLA-E, -F, -G) Class I MHC heavy chains as well as with 43-49 kDa Class I non-MHC heavy chains (CD1). b2M is expressed on nearly all nucleated cells, with neurons being a notable exception. Circulating b2M is generated during normal HLA turnover. It can also dissociate from the MHC complex and circulate as full length and N-terminal truncated peptides of 93, 91, and 90 amino acids. It has been measured in a variety of body fluids, including serum, plasma, saliva, CSF, and urine. b2M freely passes through the glomerular membrane, but it is 99% actively reabsorbed and degraded in the proximal tubule cells. Circulating b2M levels are elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions with decreased glomerular filtration. Human b2M shares 70% and 75% amino acid sequence identity with mouse and rat b2M, respectively.

Alternate Names

B2M, beta 2Microglobulin

Entrez Gene IDs

567 (Human); 12010 (Mouse); 24223 (Rat); 101867173 (Cynomolgus Monkey)

Gene Symbol

B2M

UniProt

P61769

Additional beta 2-Microglobulin Products

Product Documents for Human beta₂-Microglobulin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human beta₂-Microglobulin Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human beta2-Microglobulin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB82481

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all beta 2-Microglobulin Antibodies »

Product Summary for Human beta2-Microglobulin Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human beta2-Microglobulin Antibody

Detection of Human beta2‑Microglobulin by Western Blot.