Human beta-Arrestin 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4564
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human beta-Arrestin 2
Met1-Cys409
Accession # P32121
Met1-Cys409
Accession # P32121
Specificity
Detects human beta-Arrestin 2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human beta-Arrrestin 1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human beta-Arrestin 2 Antibody
beta-Arrestin 2 in Human Brain.
beta-Arrestin 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Human beta-Arrestin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4564) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human beta-Arrestin 2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Arrestin 2
The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 ( beta-Arrestin 1), Arrestin 3 ( beta-Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, beta-Arrestins 1 and 2 are ubiquitously expressed, and were initially described as negative regulators of G protein-coupled receptor (GPCR) signaling. More recently, beta-Arrestins have been determined to serve as scaffolds for various signaling pathways, including the MAPK cascades activating ERK2, p38 alpha, and JNK3. These beta-Arrestin scaffolds tie together the appropriate kinases in series, forming a discreet signaling module that localizes components to specific subcellular environments and facilitates greater kinase activation.
Alternate Names
ARB2, ARR2, ARRB2, betaArrestin 2
Gene Symbol
ARRB2
UniProt
Additional beta-Arrestin 2 Products
Product Documents for Human beta-Arrestin 2 Antibody
Product Specific Notices for Human beta-Arrestin 2 Antibody
For research use only
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