Human BLAME/SLAMF8 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB19072P
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 250014
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8
Ala23-Asp233
Accession # Q9P0V8
Ala23-Asp233
Accession # Q9P0V8
Specificity
Detects human BLAME/SLAMF8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SLAMF7 or rhSLAMF6 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human BLAME/SLAMF8 PE-conjugated Antibody
Detection of BLAME/SLAMF8 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes untreated (light orange filled histogram) or treated with Recombinant Human IFN-gamma (Catalog # 285-IF, dark orange filled histogram) were stained with Mouse Anti-Human BLAME/SLAMF8 PE-conjugated Monoclonal Antibody (Catalog # FAB19072P) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human BLAME/SLAMF8 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF)
Sample: Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using FAB19072P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BLAME/SLAMF8
BLAME (B-Lymphocyte Activator Macrophage Expressed), also known as SLAM Family Member 8 (SLAMF8), is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%-26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).
References
- McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
- Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
- Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.
Long Name
SLAM Family Member 8
Alternate Names
CD353, SBBI42, SLAMF8
Gene Symbol
SLAMF8
UniProt
Additional BLAME/SLAMF8 Products
Product Documents for Human BLAME/SLAMF8 PE-conjugated Antibody
Product Specific Notices for Human BLAME/SLAMF8 PE-conjugated Antibody
For research use only
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