Human peripheral blood mononuclear cells (PBMCs) gated on CD19⁺CD3^-cells were stained with Mouse Anti-Human BLIMP1/PRDM1 APC-conjugated Monoclonal Antibody (Catalog # IC36081A) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry.

U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 APC-conjugated Monoclonal Antibody (Catalog # IC36081A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human BLIMP1/PRDM1 APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: U266 human myeloma cell line and human peripheral blood mononuclear cells (PBMCs) gated on CD19⁺CD3^- cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: BLIMP1/PRDM1

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

Long Name

B Lymphocyte Induced Maturation Protein

Alternate Names

PRDM1, ZNFPR1A1

Entrez Gene IDs

639 (Human); 12142 (Mouse); 309871 (Rat)

Gene Symbol

PRDM1

UniProt

O75626

Additional BLIMP1/PRDM1 Products

Product Documents for Human BLIMP1/PRDM1 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human BLIMP1/PRDM1 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human BLIMP1/PRDM1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC36081A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all BLIMP1/PRDM1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human BLIMP1/PRDM1 APC-conjugated Antibody

Detection of BLIMP1/PRDM1 in Human PBMCs by Flow Cytometry.